Rescue of Genotype Ⅲ Velogenic Newcastle Disease Virus Strain JS/7/05/Ch

The genome of JS/7/05/Ch isolate shared more than 99% nucleotide identity with that of Mukteswar strain. However, the pathogenicity of JS/7/05/Ch was much stronger than that of Mukteswar strain. In order to provide a good foundation for the further related research, we built the rescue system of JS/7/05/Ch in this study. Based on the genomic sequence of JS/7/05/Ch strain, eight pairs of primers were designed to amplify the genomic fragments by RT-PCR and cloned into pCR2.1 vector, to construct the plasmid pJS/7/05/Ch which contained the full-length cDNA of NDV. Then the full-length cDNA was transferred to TVT7R(0.0) vector to construct full-length NDV infectious clone, pTVT/JS705, using specific enzymes. The infectious clone together with three helper plasmids (pCI-NP, pCI-P and pCI-L) were cotransfected into BSR-T7/5 cell, and the transfection supernatant was inoculated into SPF embryonated eggs to rescue the virus. The results of HA and RT-PCR indicated that JS/7/05/Ch strain of NDV was successfully rescued.     

生物防治在无公害蔬菜生产病虫害防控中的具体应用

介绍了生物防治的基本概念、指导原则、基本内容,详细阐述了生物防治在无公害蔬菜病虫害防控中的具体应用,为在无公害蔬菜生产中推行生物防治技术,确保蔬菜产品的质量安全,促进无公害蔬菜生产的健康持续发展提供理论基础。     

贵州中药材续断病虫害种类调查

2010～2012年采用定点踏查、普查和定时系统调查的方式对贵州续断中药材的病虫害种类进行了调查,并根据种植基地的病虫害发生情况,提出了续断病虫害的综合防治技术。结果表明:贵州续断共有常见病虫害13种,其中病害主要为续断根腐病(Rhizoctoniasp.)、续断根节线虫病(Meloidogyne sp.)、续断斑枯病(Septoria dipsaci)、续断病毒病、续断叶黑斑枯萎病(Alternaria sp.)和续断叶褐点斑病(Phoma sp.),虫害为斜纹夜蛾(Prodenia litura)、小地老虎(Agrotis ypsilon)、桃蚜(Myzus persicae)、油葫芦(Gryllus testaceus)、蝼蛄(Gryl-lotalpa orientali)、蛴螬(Anomala carpulenta)和螨类。     

胰腺型、经典型1型甲肝病毒对雏鸭的致病性差异

为明确胰腺型鸭1型甲肝病毒（DHAV-1）和经典型DHAV-1对不同品种雏鸭的致病性,以胰腺型DHAV-1和经典型DHAV-1分别感染7、14、21、28、35 d番鸭、半番鸭、樱桃谷鸭和麻鸭,结果表明番鸭对胰腺型DHAV-1最易感,半番鸭和樱桃谷鸭次之,麻鸭最不易感;而樱桃谷鸭对经典型DHAV-1最易感,番鸭次之;感染胰腺型DHAV-1的番鸭、半番鸭胰腺出血、明显发黄,肝脏未见出血。表明胰腺型鸭1型甲肝病毒、经典DHAV-1在易感宿主、组织嗜性和特征肉眼病变上均存在明显差异。     

Immune enhancing effects of Lactobacillus acidophilus on Newcastle disease vaccination in chickens

Introduction and purposeDespite the various vaccination programs for protection against New Castle disease, it remains an important threat to the poultry industry. The ability of the probiotic bacteria to improve the immune system in both animals and humans supports their use as immune adjuvants for vaccination. The aim of the present study was to examine the effects of Lactobacillus acidophilus in ND vaccination.Materials and methodsA total of 170 one day old chicks were divided in 5 groups. In groups A, B and C chicks were received L. acidophilus (5 × 10⁹, 3 × 10⁹ and 2 × 10⁹) and also vaccinated with inactivated and attenuated ND vaccines. In group D, chicks only vaccinated without bacterial inoculation and group E was negative control with neither vaccine nor bacteria. Then IgG and HI NDV antibody titers were measured in all tested groups.ResultsIgG and HI NDV antibody levels were significantly higher in Lactobacillus treated groups especially in group A with 5 × 10⁹ bacteria, than only vaccinated and negative control groups. Also antibody levels against NDV increased during the vaccination period especially in probiotic treated groups.ConclusionIn conclusion, L. acidophilus can use for improving immunogenicity of NDV vaccination programs.     

广东西南部水稻主要病虫发生气象规律及预测研究概述

水稻病虫害的生存、发育和繁殖甚至大暴发流行都与气象条件密切相关。本研究系统总结了2000—2018年广东省化州市水稻重大病虫害发生流行与气象条件关系及其预测模型的研究成果,分析该项目创新与突破,并与国内同类技术进行了比较,旨在为未来水稻病虫害的研究和防控工作提供重要的参考。     

鹅新城疫病毒RT-LAMP可视化检测方法的建立

本研究拟建立一种能在基层实时、便捷诊断鹅新城疫病毒（goose Newcastle disease virus，GNDV）的检测方法。根据GenBank中公布的GNDV F基因的高度同源保守序列设计特异性引物，并在反应体系中添加钙黄绿素/氯化锰指示剂，建立可视化RT-LAMP检测方法。结果显示，建立的方法能特异地检测出GNDV及NDV Lasota疫苗株，而小鹅瘟病毒（GPV）、禽流感病毒（AIV）、鸭坦布苏病毒（DTMUV）等其他病毒均为阴性。所建立的GNDV可视化RT-LAMP检测方法对RNA的最低检测限为10 pg，反应过程不需PCR仪等复杂的仪器，50 min即可完成反应，可经肉眼观察反应体系颜色判定结果。该方法特异性强、灵敏度高，且操作安全、简便、快捷，可满足基层筛查GNDV的需求。